Mendeley
TY  _  JOUR
ID  -  1402060443763
TI  -  Isolation and screening of pectinase producing Aspergillus niger and use of whey to optimize enzyme production
JO  -  Knowledge of  Microbiology
JA  -  ES
LA  -  fa
SN  -  
AU  -  pouya Mahsa
AU  -  Ghazi shokoofeh
AU  -  Tukmechi Amir
AD  -  دانشگاه آزاد اسلامی- علوم پزشکی تهران
AD  -  دانشگاه آزاد اسلامی- علوم پزشکی تهران
AD  -  دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران
Y1  -  2023
PY  -  2023
VL  -  1
IS  -  
SP  -  97
EP  -  106
KW  -  Aspergillus niger
KW  -   Pectinase enzyme
KW  -   DNS method
KW  -   Whey
DO  -  
N2  -  Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and applications of this enzyme in food processing, agriculture, the purpose of this research is isolating and screening pectinase-producing fungi from some of rotten fruits and vegetables, as well as optimizing the enzyme production condition.
Materials and methods: Isolation and identification of pectinase producing fungi from rotten fruits (peach, plum and apple) and vegetables (onion) were done. Isolation and screening the most capable fungal strains producing pectinase was used by cultivation on specific Pectin Agar medium, staining with lactophenol cotton blue and slide culture method.
In order to identify the isolated fungi more accurately, the molecular technique like 18S rRNA sequencing was used. Optimizing the production rate of pectinase enzyme carried out using pectin substrate accompanied by different Lactic Whey and Permeate whey under different temperatures and pH levels, and the activity of enzyme was measured by the standard DNS method.
Results: one Aspergillus niger fungus producing pectinase enzyme was isolated from onion. Results for optimization conditions shows that the identified strain, shows an enzyme activity equal to 103/018 (IU/ml) in the presence of permeate whey and pectin substrate, under incubation at 25 C° ,140 rpm, and pH 6.5. While, pectinase production with only pure pectin substrate was lower equal to 92.717 (IU/ml) when incubation at 25 C° , same rpm and pH  7. 
Conclusions: Potent native fungal strains isolated from our country are suitable candidates for the production of important industrial enzymes such as pectinase. Also using cost effective substrates like Whey can be replaced by expensive pure substrates in production industry for the important enzymes production process.

UR  - rimag.ir/en/Article/43763
L1  - rimag.ir/en/Article/Download/43763
ER  -  
